| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Differential gene expression after total replacement of dietary fish meal and fish oil by plant products in rainbow trout (Oncorhynchus mykiss) liver
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| Autores: |
Panserat, S.
Hortopan, G. A.
Plagnes-juan, E.
Kolditz, C.
Lansard, M.
Skiba-cassy, S.
Esquerre, D.
Geurden, I.
Medale, Francoise
Kaushik, Sadasivam
Corraze, Genevieve
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| Data: |
2009-09
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| Ano: |
2009
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| Palavras-chave: |
Rainbow trout
Transcriptome
Metabolism
Liver
Vegetable products
Marine ingredients
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| Resumo: |
Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100% plant-based diet (0% FM, 0% FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A large majority of differentially expressed genes were involved in metabolism (57%) and the others in cellular processes (21%) and transport (10%). Among the genes involved in metabolism (n = 86), 37% were associated with protein metabolism (proteolysis, amino acid catabolism), 21% with lipid metabolism (fatty acid biosynthesis, cholesterol biosynthesis), 30% with nucleic acid metabolism and 8% with glucose metabolism. Specifically, we found in rainbow trout fed the 100% plant diet an over-expression of genes involved in lipid biosynthesis (cholesterol metabolism and desaturation of polyunsaturated fatty acids) and an over-expression of a new metabolic actor, i.e., glycerol kinase which plays a key role at the interface of glucose-lipid metabolism. Overall, these data demonstrate that a number of intermediary metabolic effects occur in trout fed a totally plant-based diet. (C) 2009 Elsevier B.V. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2009/publication-6684.pdf
DOI:10.1016/j.aquaculture.2009.05.013
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/6684/
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| Formato: |
application/pdf
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| Fonte: |
Aquaculture (Elsevier), 2009-09 , Vol. 294 , N. 1-2 , P. 123-131
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| Direitos: |
2009 Elsevier Ltd All rights reserved.
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